Continue on overlap and restrictions of linear plasmid and plasma extract
This week for the lab we worked with our amplified sample of overlap. We made a 1.2% gel which is 3.6g of agarose and 30mL of TAE buffer. When we placed our product in the wells we used 10 ul of sample and 2ul of dye not using PCR water because we wanted a better view of what we had in our sample. In this photo, you'll see in the second row our 6ul ladder and our fourth row is the 12ul sample in the gel. We ran that gel and it took the whole lab session so we worked on extracting plasma from Ecoli that was grown over the weekend. So we can have more tet since we already have a lot of left and right. We used the molecular biology Thermo scientific GeneJET Plasmid Miniprep kit. The website we used to follow the directions is thermofisher.com/studio first step was we resuspended the pelleted cells in 250ul of resuspension solution and pipetted up and down til we no longer had cell clumps. Then add 250ul of the lysis solution and 350ul of the neutralization solution, flipped ups...
